Isolation of Mannanase-producing Bacteria, Bacillus subtilis WL-6 and WL-11, and Cloning and Characterization of Mannanase
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چکیده
منابع مشابه
Isolation, Purification and Characterization of Mannanase from Bacillus subtilis MAN-511
A bacterium, MAN-511 that produced extracellular mannanase, was isolated and identified as Bacillus subtilis on the basis of 16S rDNA phylogenetic analysis. The enzyme was purified to apparent homogeneity by precipitasi ammonium sulfat 70% and Sephadex G-75 chromatography procedures. The mannanase was purified 7.6 fold and specificity of 9.3 U/mg protein. SDS-PAGE of the purified enzyme showed ...
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MANB36, a secrete endo-1,4-d-mannanase produced by Bacillus subtilis B36, was purified to homogeneity from a culture supernatant and characterized. The optimum pH value for the mannanase activity of MANB36 is 6.4 and the optimum temperature is 50 ∞C. The enzyme activity of MANB36 is remarkably thermostable at 60 ∞C and the specific activity of MANB36 is 927.84 U/mg. Metal cations (except Hg2+ a...
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متن کاملGene cloning, expression, and characterization of a novel beta-mannanase from Bacillus circulans CGMCC 1416.
A DNA fragment containing 2,079 base pairs from Bacillus circulans CGMCC 1416 was cloned using degenerate PCR and inverse PCR. An open reading frame containing 981 bp was identified that encoded 326 amino acids residues, including a putative signal peptide of 31 residues. The deduced amino acid sequence showed the highest identity (68.1%) with endo-beta-1,4-D-mannanase from Bacillus circulans s...
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ژورنال
عنوان ژورنال: Journal of Life Science
سال: 2016
ISSN: 1225-9918
DOI: 10.5352/jls.2016.26.10.1113